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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/25967
Title: Clonal dissemination of Vibrio parahaemolyticus displaying similar DNA fingerprint but belonging to two different serovars (03:K6 and 04:K68) in Thailand and India
Authors: N. R. Chowdhury
S. Chakraborty
B. Eampokalap
W. Chaicumpa
M. Chongsa-Nguan
P. Moolasart
R. Mitra
T. Ramamurthy
S. K. Bhattacharya
M. Nishibuchi
Y. Takeda
G. Balakrish Nair
National Institute of Cholera and Enteric Diseases India
Bamrasnaradura Infectious Disease Institute
Mahidol University
Kyoto University
National Institute of Infectious Diseases
Keywords: Immunology and Microbiology;Medicine
Issue Date: 26-Oct-2000
Citation: Epidemiology and Infection. Vol.125, No.1 (2000), 17-25
Abstract: Active surveillance of Vibrio parahaemolyticus infection among hospitalized patients in Calcutta, India, showed the appearance of the O4:K68 serovar for the first time in March 1998 alongside the continued predominant incidence of the O3:K6 serovar. Strains belonging to both these serovars have been reported to possess pandemic potential. The genomes of O3:K6 and O4:K68 strains and for comparison, non-O3:K6 and non-O4:K68 strains isolated from two different countries, India and Thailand, were examined by different molecular techniques to determine their relatedness. The O3:K6 and O4:K68 strains from Calcutta and Bangkok carried the tdh gene but not the trh gene. Characterization of representative strains of these two serovars by ribotyping and by arbitrarily primed-polymerase chain reaction (AP-PCR) showed that the isolates had identical ribotype and DNA fingerprint. Pulsed-field gel electrophoresis (PFGE) performed with the same set of strains yielded nearly similar restriction fragment length polymorphism (RFLP) patterns for the O3:K6 and O4:K68 isolates from Calcutta and Thailand. Phylogenetic analysis of the NotI RFLP showed that the O3:K6 and O4:K68 strains formed a cluster with 78-91% similarity thus indicating close genetic relationship between the two different serovars isolated during the same time-frame but from widely separated geographical regions. The non-O3:K6 and non-O4:K68, in contrast, showed different ribotype, AP-PCR and PFGE patterns.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0033770075&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/25967
ISSN: 09502688
Appears in Collections:Scopus 1991-2000

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