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Title: Differential diagnosis of schistosomiasis mekongi and trichinellosis in human
Authors: Wannaporn Ittiprasert
Piyarat Butraporn
Viroj Kitikoon
Kosoom Klongkamnuankarn
Khamlien Pholsena
Viengxay Vanisaveth
Yuwaporn Sakolvaree
Manas Chongsa-Nguan
Pramuan Tapchaisri
Yuvadee Mahakunkijcharoen
Hisao Kurazono
Hideo Hayashi
Wanpen Chaicumpa
Mahidol University
Institute of Malaria, Parasitic Diseases and Entomology
University of Tsukuba
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Sep-2000
Citation: Parasitology International. Vol.49, No.3 (2000), 209-218
Abstract: An indirect (plate) ELISA and, a more convenient version, a dot-blot (membrane) ELISA have been developed using haemocyanin of a mollusk, Megathura crenulata, i.e. keyhole limpet haemocyanin (KLH) and purified, specific antigen of Trichinella spiralis (APTsAg) obtained from a monoclonal antibody-affinity column chromatography, for differential diagnosis of schistosomiasis mekongi and trichinellosis. Serum samples of patients with parasitologically confirmed trichinellosis were reactive to both antigens in both versions of ELISA while sera of patients with schistosomiasis mekongi were positive only to the KLH. Both ELISA were negative when used to test sera of normal controls and patients with gnathostomiasis, paragonimiasis and opisthorchiasis. (C) 2000 Elsevier Science Ireland Ltd.
ISSN: 13835769
Appears in Collections:Scopus 1991-2000

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