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Title: Genetic characterization of incident HIV type 1 subtype E and B strains from a prospective cohort of injecting drug users in Bangkok, Thailand
Authors: Shambavi Subbarao
Suphak Vanichseni
Dale J. Hu
Dwip Kitayaporn
Kachit Choopanya
Suwanee Raktham
Nancy L. Young
Chantapong Wasi
Ruengpung Sutthent
Chi Cheng Luo
Artur Ramos
Timothy D. Mastro
Centers for Disease Control and Prevention
Bangkok Metropolitan Administration
Mahidol University
HIV/AIDS Collaboration
Thailand Ministry of Public Health
Keywords: Immunology and Microbiology;Medicine
Issue Date: 20-May-2000
Citation: AIDS Research and Human Retroviruses. Vol.16, No.8 (2000), 699-707
Abstract: We obtained specimens from 128 HIV-1 seroconverters identified from 1995 through 1998 in a prospective cohort study of 1209 HIV-negative injecting drug users (IDUs) in Bangkok, Thailand. Epidemiologic data indicated that parenteral transmission accounted for nearly all infections. HIV-1 DNA from the C2-V4 env region was sequenced, and phylogenetic analyses determined that 102 (79.7%) of the specimens were subtype E and 26 (20.3%) subtype B strains. All subtype B strains clustered with strains often referred to in previous studies as Thai B or B'. The interstrain nucleotide distance (C2-V4) within subtype E strains was low (mean, 6.8%), and pairwise comparisons with a prototype subtype E strain, CM244, showed limited divergence (mean, 5.6%). The subtype B stains showed greater interstrain divergence (mean, 9.2%) and were significantly divergent from the prototype B strain HIV-MN (mean, 13.0%; p < 0.0001). The subtype E strains had significantly lower mean V3 loop charge than did subtype B strains (p = 0.017) and, on the basis of analysis of amino acid sequences, were predicted to be predominantly (91%) non- syncytium-inducing (NSI), chemokine coreceptor CCR5-using (CCR5+) viruses. The subtype B strains had a higher mean V3 loop charge, and a smaller proportion (23%) were predicted to be NSI/CCR5+ viruses. This study demonstrates that most incident HIV-1 infections among Bangkok IDUs are due to subtype E viruses, with a narrow spectrum of genetic diversity. The characterization of incident HIV-1 strains from 1995 to 1998 will provide important baseline information for comparison with any breakthrough infections that occur among IDUs in Bangkok who are participating in an HIV-1 vaccine efficacy trial initiated in 1999.
ISSN: 08892229
Appears in Collections:Scopus 1991-2000

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