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Title: Detection of poliovirus, hepatitis A virus and rotavirus from sewage and water samples
Authors: Leera Kittigul
Boonyos Raengsakulrach
Sontana Siritantikorn
Raweewan Kanyok
Fuangfa Utrarachkij
Pornpan Diraphat
Vipa Thirawuth
Kanokrat Siripanichgon
Supornvit Pungchitton
Kitja Chitpirom
Nattasai Chaichantanakit
Kanda Vathanophas
Mahidol University
Armed Forces Research Institute of Medical Sciences, Thailand
Keywords: Medicine
Issue Date: 1-Mar-2000
Citation: Southeast Asian Journal of Tropical Medicine and Public Health. Vol.31, No.1 (2000), 41-46
Abstract: A modified adsorption-elution technique for concentration of enteric viruses from sewage and water samples was developed. The viruses in water were concentrated by negatively charged membrane filtration, eluted with 2.9% tryptose phosphate broth containing 6% glycine pH 9.0, and reconcentrated using centrifugation by a speedVac concentrator. The presence of poliovirus, hepatitis A virus (HAV) RNA, and rotavirus antigen was determined by cell culture isolation, nested polymerase chain reaction (nested PCR), and enzyme-linked immunosorbent assay (ELISA), respectively. A total of 100 sewage and water samples were collected from various sources in congested communities in Bangkok, concentrated and examined for those enteric viruses. Of 20 surface water samples from canals which located near sewage drains, 15% were positive for HAV RNA by nested PCR. Of 48 domestic sewage samples from man-holes of underground sewers, 8% were positive for rotavirus antigen by ELISA. Even though the samples were concentrated 256-2,000 fold, poliovirus was not found by isolation in cell culture.
ISSN: 01251562
Appears in Collections:Scopus 1991-2000

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