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|Title:||A human volunteer challenge model using frozen bacteria of the new epidemic serotype, V. cholerae O139 in Thai volunteers|
Mitchell B. Cohen
Gilbert M. Schiff
University of Cincinnati
Organisation Mondiale de la Sante
National Institute of Allergy and Infectious Diseases
|Keywords:||Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology;Medicine;Veterinary|
|Citation:||Vaccine. Vol.20, No.5-6 (2001), 920-925|
|Abstract:||A total of 35 volunteers were recruited for an IRB-approved inpatient dose-escalation challenge. The goal was to identify a dose that produced an observed cholera attack rate ≥80% and an illness of sufficient severity during the defined study period such that the model would be useful for determining vaccine protection. Volunteers were challenged in groups of 5 with V. cholerae O139 that had been reconstituted immediately before use. Only 2 out of 5 volunteers who received the lowest dose (4.3 × 104cfu) had diarrhea. As the inoculum size increased, the attack rate of diarrhea increased to 3-4 of 5 volunteers. At the highest dose tested, approximately 5 × 107cfu, the attack rate was 73%. We recommend the use of frozen V. Cholera O139 in a human experimental challenge model to assess cholera vaccine efficacy (VE) in a cholera endemic area but with 4 days observation period before initiation of tetracycline to allow assessment of severity. © 2001 Elsevier Science Ltd. All rights reserved.|
|Appears in Collections:||Scopus 2001-2005|
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