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Title: Structural and promoter regions of the murine pyruvate carboxylase gene
Authors: Sarawut Jitrapakdee
Nathida Petchamphai
Piyanate Sunyakumthorn
John C. Wallace
Vichai Boonsaeng
Mahidol University
University of Adelaide
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 21-Sep-2001
Citation: Biochemical and Biophysical Research Communications. Vol.287, No.2 (2001), 411-417
Abstract: We have cloned and sequenced the gene encoding mouse pyruvate carboxylase (mPC) [EC]. The coding region contains 19 exons, one 5′-untranslated region exon, and 19 introns in 22 kb of genomic DNA. This gene's exon/intron organization is highly conserved with respect to rat and human PC genes. The mPC gene promoter lacks canonical TATA and CCAAT boxes, in common with a number of housekeeping genes. Transient expressions in COS-1 of a luciferase reporter gene under the control of 5′-nested deletions of the 5′-flanking sequence of the mPC gene have identified the 166-bp minimal sequence required for basal transcription. Alternative splicing at the 5′-untranslated region exon of the mouse PC gene results in the production of two alternate transcripts bearing different 5′-noncoding regions. Both transcripts are highly expressed in kidney and liver and moderately expressed in heart and testis and expressed at a low level in spleen. © 2001 Academic Press.
ISSN: 0006291X
Appears in Collections:Scopus 2001-2005

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