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Title: Lighting E. coli cells as biological sensors for Cd2+
Authors: Virapong Prachayasittikul
Chartchalerm Isarankura Na Ayudhya
Leif Bulow
Mahidol University
Lunds Universitet
Keywords: Biochemistry, Genetics and Molecular Biology;Chemical Engineering;Immunology and Microbiology
Issue Date: 6-Sep-2001
Citation: Biotechnology Letters. Vol.23, No.16 (2001), 1285-1291
Abstract: Whole cells of E. coli expressing a chimeric cadmium-binding peptide fused to green fluorescent protein (CdBPGFP) were prepared and applied for the determination of cadmium. Construction of the structural gene was performed by inserting two synthetic oligonucleotides coding for four repeats of a Cd-binding peptide (His-Ser-Gln-Lys-Val-Phe) into the 5′-end of the GFPuv gene. Similarly, a hexahistidine-green fluorescent protein (his6GFPuv) was prepared and used as a reference in the determinations of heavy metals. The lowest concentrations of Cd, which activated the fluorescence, were 0.5 μM, 50 μM, and 0.5 mM for cells carrying CdBP4GFP, his6GFP and native GFP, respectively.
ISSN: 01415492
Appears in Collections:Scopus 2001-2005

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