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dc.contributor.authorS. Thamthiankulen_US
dc.contributor.authorS. Suan-Ngayen_US
dc.contributor.authorS. Tantimavanichen_US
dc.contributor.authorW. Panbangreden_US
dc.contributor.otherMahidol Universityen_US
dc.identifier.citationApplied Microbiology and Biotechnology. Vol.56, No.3-4 (2001), 395-401en_US
dc.description.abstractThe chitinase gene (chiA71) from Bacillus thuringiensis subsp, pakistani consists of an open reading frame of 1,905 nucleotides encoding 635 amino acid residues with an estimated molecular mass of 71 kDa. Comparison of the deduced amino acid sequence of the mature enzyme to other microbial chitinases shows a putative catalytic domain and a region with conserved amino acids similar to that of the type III module of fibronectin and a chitin-binding domain. By activity detection of chitinase on SDS-PAGE after renaturation, the molecular mass of protein bands with chitinase activity were 66, 60, 47, and 32 kDa. The N-terminal amino acid sequence of each chitinase activity band was the same (Asp-Ser-Pro-Lys-Gln), suggesting that the 60-, 47-, and 32-kDa chitinases were derived from the 66-kDa chitinase by processing step(s) at the C-terminus. The enzyme was identified as an exochitinase, since it generated N-acetylglucosamine from early stage of colloidal chitin hydrolysis. The crude protein (2.3-18.4 mg/ml), containing chitinase at final activities of 8, 16, 32, and 64 mU/ml, was toxic to Aedes aegypti larvae and caused mortalities of 7.5, 15.0, 51.3, and 70.0% respectively, but the same amount of crude protein from a B. thuringiensis subsp. pakistani mutant lacking chitinase was not toxic.en_US
dc.rightsMahidol Universityen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleChitinase from Bacillus thuringiensis subsp. pakistanien_US
Appears in Collections:Scopus 2001-2005

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