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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/26562
Title: Molecular cloning and characterization of cathepsin L encoding genes from Fasciola gigantica
Authors: Rudi Grams
Suksiri Vichasri-Grams
Prasert Sobhon
E. Suchart Upatham
Vithoon Viyanant
Thammasat University
Mahidol University
Burapha University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 12-Jul-2001
Citation: Parasitology International. Vol.50, No.2 (2001), 105-114
Abstract: In this study cDNAs encoding cathepsin L-like proteins of Fasciola gigantica were cloned by the reverse transcription polymerase chain reaction method (RT-PCR) from total RNA of adult specimens. DNA sequence analyses revealed that six different cathepsin L cDNA fragments were isolated, which have DNA sequence identities of 87-99% towards the homologous genes from F. hepatica. Gene expression was studied at the RNA level by Northern and RNA in situ hybridizations. Northern analysis showed the cathepsin L genes to be strongly expressed in adult parasites as a group of 1050 nt sized RNAs. RNA in situ hybridization localized cathepsin L RNA to the cecal epithelial cells. Southern hybridization was used to determine the number of cathepsin L genes and indicated the presence of a family of closely related cathepsin L genes in the genome of F. gigantica. Copyright © 2001 Elsevier Science Ireland Ltd.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0034950247&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/26562
ISSN: 13835769
Appears in Collections:Scopus 2001-2005

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