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|Title:||Molecular cloning and characterization of cathepsin L encoding genes from Fasciola gigantica|
E. Suchart Upatham
|Keywords:||Immunology and Microbiology;Medicine|
|Citation:||Parasitology International. Vol.50, No.2 (2001), 105-114|
|Abstract:||In this study cDNAs encoding cathepsin L-like proteins of Fasciola gigantica were cloned by the reverse transcription polymerase chain reaction method (RT-PCR) from total RNA of adult specimens. DNA sequence analyses revealed that six different cathepsin L cDNA fragments were isolated, which have DNA sequence identities of 87-99% towards the homologous genes from F. hepatica. Gene expression was studied at the RNA level by Northern and RNA in situ hybridizations. Northern analysis showed the cathepsin L genes to be strongly expressed in adult parasites as a group of 1050 nt sized RNAs. RNA in situ hybridization localized cathepsin L RNA to the cecal epithelial cells. Southern hybridization was used to determine the number of cathepsin L genes and indicated the presence of a family of closely related cathepsin L genes in the genome of F. gigantica. Copyright © 2001 Elsevier Science Ireland Ltd.|
|Appears in Collections:||Scopus 2001-2005|
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