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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/26617
Title: Affinity purified oval antigen for diagnosis of Opisthorchiasis Viverrini
Authors: Thitima Wongsaroj
Yuwaporn Sakolvaree
Wanpen Chaicumpa
Wanchai Maleewong
Viroj Kitikoon
Pramuan Tapchaisri
Manas Chongsa-nguan
John H. Cross
Mahidol University
Keywords: Medicine
Issue Date: 1-Dec-2001
Citation: Asian Pacific Journal of Allergy and Immunology. Vol.19, No.4 (2001), 245-258
Abstract: Monoclonal antibodies (MAb) were raised against an oval antigen of the liver fluke Opisthorchis viverrini which is the causative agent of a parasitosis, i.e. opisthorchiasis in Thailand. The antibodies were used in an affinity column to purify the O. viverrini oval antigen from a crude extract of adult parasites by chromatography. The oval antigen was then used in a membrane (dot) ELISA for detecting antibodies in serum samples of parasitologically confirmed Opisthorchis viverrini infected individuals (adult parasites were found in stools after praziquantel treatment and salt purgation), as well as of individuals infected with other parasites and parasite-free controls. The MAb-based dot-ELISA using the affinity purified O. viverrini oval antigen revealed 100% sensitivity, specificity and accuracy for detecting O. viverrini infection. The test is simple, rapid and highly reproducible. Several samples can be tested at the same time without the requirement for special equipment or much increase in testing time; thus it is suitable for mass screening for O. viverrini exposure, especially in new endemic areas. Furthermore using serum specimens could increase patient and community compliance compared to the conventional parasitological survey which uses stool samples for the detection of O. viverrini ova, without treatment and subsequent salt purgation, this conventional method shows a low sensitivity and is also unpleasant to both the sample donors and the laboratory technicians which has historically shown a further negative impact on the final outcome.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0035733851&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/26617
ISSN: 0125877X
Appears in Collections:Scopus 2001-2005

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