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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/26630
Title: Diagnosis of intestinal amebiasis using salivary IgA antibody detection
Authors: Paibul Punthuprapasa
Nitaya Thammapalerd
Udomporn Chularerk
Kravee Charoenlarp
Manoon Bhaibulaya
Faculty of Medicine, Siriraj Hospital, Mahidol University
Mahidol University
Keywords: Medicine
Issue Date: 1-Dec-2001
Citation: Southeast Asian Journal of Tropical Medicine and Public Health. Vol.32, No.SUPPL. 2 (2001), 159-164
Abstract: Attempts were made to use soluble antigen extract of strain HK-9 of Entamoeba histolytica to detect salivary IgA antibodies in intestinal amebiasis patients by using ELISA. Total salivary samples of 109 individuals were divided into four groups. Group I comprised 32 patients whose stools were positive only for E. histolytica cysts and/or trophozoites. Group II comprised 12 individuals whose stools were positive for E. histolytica and other intestinal parasites. Group III comprised 36 individuals whose stools were negative for E. histolytica but contained other intestinal parasites such as E. coli, E. nana, Blastocystis hominis, Trichomonas hominis. Giardia lamblia. Opisthorchis viverrini, and hookworm. Group IV comprised 29 healthy individuals whose stools were free from any intestinal parasitic infections. Based on the mean optical density, OD + 2SD of the results from 29 parasitologically negative healthy individuals, the cut-off OD value for salivary IgA antibodies was 1.265. Therefore, the assays were positive in 14 out of 32 (43.75%) of group 1 and 2 out of 12 (16.6%) of group II. The assays were positive in 16 out of 36 (44.44%) for group III whereas 2 out of 29 (6.90%) for group IV were positive. The overall sensitivity and specificity of the assays were 36% and 72%, respectively. The false positive rate was 28% and the false negative rate was 64%. The predictive values of positive and negative results were 47% and 63%, respectively. The diagnostic accuracy of ELISA for the presence of salivary IgA antibodies was 58%.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0042832571&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/26630
ISSN: 01251562
Appears in Collections:Scopus 2001-2005

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