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Title: Parvolike virus in the hepatopancreas of freshwater prawns macrobrachium rosenbergii cultivated in Thailand
Authors: Warachin Gangnonngiw
Wansika Kiatpathomchai
Siriporn Sriurairatana
Kesinee Laisutisan
Niti Chuchird
Chalor Limsuwan
Timothy W. Flegel
Mahidol University
Thailand National Science and Technology Development Agency
Kasetsart University
Keywords: Agricultural and Biological Sciences
Issue Date: 19-Aug-2009
Citation: Diseases of Aquatic Organisms. Vol.85, No.3 (2009), 167-173
Abstract: A survey of cultivated giant freshwater prawns Macrobrachium rosenbergii from Thailand revealed the presence of unusual spherical to ovoid inclusions in nuclei of hepatopancreas tubule epithelial cells. These began as small eosinophilic inclusions that became more basophilic as they increased in size. They were present in both R-cells and E-cells but were largest and deeply basophilic only in the E-cells. Confocal laser microscopy revealed that stained nucleic acid fluorescence from the inclusions was lost by treatment with DNase I specific for double- and single-stranded DNA and also lost or reduced by treatment with mungbean nuclease specific for single-stranded nucleic acids. Transmission electron microscopy (TEM) revealed that the inclusions contained tightly packed, unenveloped, viral-like particles of approximately 25 to 30 nm diameter, resembling those produced by shrimp parvoviruses. However, PCR, in situ hybridization and immunohistochemical tests for shrimp parvoviruses previously reported from Thailand were all negative. These results suggested that the inclusions contained a parvo-like virus, not previously reported from M. rosenbergii in Thailand. © Inter-Research 2009.
ISSN: 16161580
Appears in Collections:Scopus 2006-2010

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