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Title: Characterization and multiplex genotyping of 16 polymorphic microsatellite loci in the endangered bumble-bee bat, Craseonycteris thonglongyai (Chiroptera: Craseonycteridae)
Authors: Sébastien J. Puechmaille
Piyathip Piyapan
Medhi Yokubol
Meriadeg Ar Gouilh
Khin Mie Mie
Paul J. Bates
Chutamas Satasook
Tin Nwe
Si Si Hla Bu
Iain MacKie
Emma C. Teeling
University College Dublin
Prince of Songkla University
Museum National d'Histoire Naturelle
Mahidol University
Yangon University
Harrison Institute
Mandalay University
University of Aberdeen
Keywords: Agricultural and Biological Sciences;Biochemistry, Genetics and Molecular Biology
Issue Date: 1-Aug-2009
Citation: Conservation Genetics. Vol.10, No.4 (2009), 1073-1076
Abstract: The bumble-bee bat (Craseonycteris thonglongyai) is an endangered species with a limited distribution range and a suspected declining population. Of the 30 microsatellites developed and tested in C. thonglongyai, 16 were amplified in two multiplex polymerase chain reactions (PCRs). Based on genotypes of 64 individuals from two populations, expected heterozygosities, averaged 0.50 in Myanmar and 0.61 in Thailand. Only one pair of loci showed linkage disequilibrium in one population and no deviation from Hardy-Weinberg equilibrium was detected. This set of 16 microsatellites will be useful to study population structure and gene flow in C. thonglongyai. © 2008 Springer Science+Business Media B.V.
ISSN: 15729737
Appears in Collections:Scopus 2006-2010

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