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|Title:||Persistent expression of shrimp-virus antigens in two insect cell lines challenged with two shrimp viruses|
Timothy W. Flegel
Rajamangala University of Technology Tawon-ok
National Science Technology Development Agency
|Keywords:||Agricultural and Biological Sciences|
|Citation:||Fish Pathology. Vol.44, No.2 (2009), 86-93|
|Abstract:||Experiments with crustacean viruses are hampered by lack of susceptible continuous cell lines. To overcome this problem, immortal mosquito and lepidopteran cell lines were both separately challenged with a shrimp DNA virus (white spot syndrome virus: WSSV, = PRDV) and RNA virus (yellow head virus: YHV) followed by serial, split-passage with immunohistochemical monitoring by confocal laser microscopy using labeled monoclonal antibodies to shrimp viral antigens. Stable, immortal cultures with 100% of the cells expressing shrimp-virus antigens were obtained, although the infected cells appeared grossly normal by phase contrast microscopy. Nor did they show any ultrastructural modifications characteristic of the challenge viruses. These persistently-expressing insect cell cultures were stable and could be continuously passaged, stored and revived as required. Since disparate viruses and insect cells were used, this appears to be a generic process that may be applicable to other shrimp viruses as well. © 2009 The Japanese Society of Fish Pathology.|
|Appears in Collections:||Scopus 2006-2010|
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