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dc.contributor.authorSurintorn Boonanuntanasarnen_US
dc.contributor.authorSakol Panyimen_US
dc.contributor.authorGoro Yoshizakien_US
dc.contributor.otherSuranaree University of Technologyen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherNational University Corporation Tokyo University of Marine Science and Technologyen_US
dc.identifier.citationTransgenic Research. Vol.18, No.3 (2009), 323-325en_US
dc.description.abstractWe conducted in vitro transcription activities of the three zebrafish U6 putative promoters across species in cell extracts prepared from Nile tilapia (Oreochromis niloticus) and shrimps. The transcription efficiency of these putative U6 promoters in Nile tilapia cell extracts was similar to that of zebrafish cell extracts. In addition, all three zebrafish U6 snRNA promoters were able to express the shRNA in cell extracts prepared from two shrimp species, Penaeus monodon and Litopenaeus vannamei. However, the shRNA transcription products in shrimp cell extracts showed weaker signals. These U6 promoters could promote shRNA expression, suggesting that they have the potential for use for vector-based RNAi in Nile tilapia and shrimps. A putative U6 promoter would provide a powerful tool for long-term GKD in these aquaculture-related species. © 2009 Springer Science+Business Media B.V.en_US
dc.rightsMahidol Universityen_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleUsage of putative zebrafish U6 promoters to express shRNA in Nile tilapia and shrimp cell extractsen_US
Appears in Collections:Scopus 2006-2010

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