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Title: Simultaneous detection of pediocin gene and species differentiation between Pediococcus acidilactici and P. pentosaceus in a one-step multiplex-overlapping PCR method
Authors: Duongdearn Suwanjinda
Kittipong Pala-or
Watanalai Panbangred
Mahidol University
Keywords: Agricultural and Biological Sciences;Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology
Issue Date: 1-Apr-2009
Citation: Food Biotechnology. Vol.23, No.2 (2009), 179-189
Abstract: We developed a one-step method based on multiplex-overlapping polymerase chain reaction (mPCR) for species differentiation of Pediococcus acidilactici and P. pentosaceus as well as simultaneous detection of pediocin (ped) gene. The mPCR was performed in a single reaction tube with six primers consisting of four primers for identification of P. acidilactici and P. pentosaceus and two primers for pediocin gene amplification. We distinguished both species from nine other species of pediococci as well as several other lactic acid bacteria (LAB). The identification performed by this mPCR was confirmed by partial 16S rDNA sequencing of selected pediococcal and LAB isolates. This multiplex-overlapping PCR is a simple, rapid determination and reliable method for differentiation of both pediococcal species as well as determination of the ability to produce pediocin in a single PCR reaction. © Taylor & Francis Group, LLC.
ISSN: 15324249
Appears in Collections:Scopus 2006-2010

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