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Title: Leptospirosis research: Fast, easy and reliable enumeration of mobile leptospires
Authors: Stefan Schreier
Wannapong Triampo
Galayanee Doungchawee
Darapond Triampo
Sudarat Chadsuthi
Hochschule München
Mahidol University
Keywords: Agricultural and Biological Sciences;Biochemistry, Genetics and Molecular Biology
Issue Date: 1-Jan-2009
Citation: Biological Research. Vol.42, No.1 (2009), 5-12
Abstract: Leptospirosis caused by Leptospira interrogans is the most widespread zoonosis and a major public health problem worldwide. Based on light-scattering and absorption, quantification of leptospires using UV-VIS spectroscopy was used as an indirect counting technique by measuring the optical density and comparing this to automated direct counting using a counting chamber in combination with imaging and analyzing software. Two serovars, Bangkok and Copenhagenii, from log-phase growth were used for the establishment of standard curves. They were found to be linear and slightly different in gradient for each serovar. The ease, rapidity, and reliability of these two adapted and optimized counting techniques may provide a useful alternative enumeration technique for leptospirosis research.
ISSN: 07176287
Appears in Collections:Scopus 2006-2010

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