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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/27143
Title: Direct stimulation of the transcellular and paracellular calcium transport in the rat cecum by prolactin
Authors: Kamonshanok Kraidith
Walailuk Jantarajit
Jarinthorn Teerapornpuntakit
La Iad Nakkrasae
Nateetip Krishnamra
Narattaphol Charoenphandhu
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Medicine
Issue Date: 1-Sep-2009
Citation: Pflugers Archiv European Journal of Physiology. Vol.458, No.5 (2009), 993-1005
Abstract: Prolactin (PRL) is reported to stimulate calcium absorption in the rat's small intestine. However, little is known regarding its effects on the cecum, a part of the large intestine with the highest rate of intestinal calcium transport. We demonstrated herein by quantitative real-time polymerase chain reaction and Western blot analysis that the cecum could be a target organ of PRL since cecal epithelial cells strongly expressed PRL receptors. In Ussing chamber experiments, PRL enhanced the transcellular cecal calcium absorption in a biphasic dose-response manner. PRL also increased the paracellular calcium permeability and passive calcium transport in the cecum, which could be explained by the PRL-induced decrease in transepithelial resistance and increase in cation selectivity of the cecal epithelium. PRL actions in the cecum were abolished by inhibitors of phosphoinositide 3-kinase (PI3K), protein kinase C (PKC), and RhoA-associated coiled-coil forming kinase (ROCK), but not inhibitors of gene transcription and protein biosynthesis. In conclusion, PRL directly enhanced the transcellular and paracellular calcium transport in the rat cecum through the nongenomic signaling pathways involving PI3K, PKC, and ROCK. © 2009 Springer-Verlag.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=68149094240&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/27143
ISSN: 14322013
00316768
Appears in Collections:Scopus 2006-2010

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