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dc.contributor.authorThichakorn Jittawuttipokaen_US
dc.contributor.authorSarinya Buranajitpakornen_US
dc.contributor.authorMayuree Fuangthongen_US
dc.contributor.authorHerbert P. Schweizeren_US
dc.contributor.authorPaiboon Vattanaviboonen_US
dc.contributor.authorSkorn Mongkolsuken_US
dc.contributor.otherChulabhorn Research Instituteen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherChulabhorn Graduate Instituteen_US
dc.contributor.otherColorado State Universityen_US
dc.identifier.citationFEMS Microbiology Letters. Vol.298, No.1 (2009), 111-117en_US
dc.description.abstractTransposon mini-Tn7 vectors insert into the chromosome of several Gram-negative bacteria in a site-specific manner. Here, we showed the application of mini-Tn7 as single copy site-specific integration vector system for Xanthomonas campestris pv. campestris. The transposition of the mini-Tn7 into the bacterial genome was detected at a Tn7 attachment (attTn7) site located downstream of glmS1. Furthermore, using a newly constructed vector pBBR1FLP2 containing the flipase (FLP) recombinase for site-specific excision of the sequence between the FLP recombinase target (FRT) sites, and a sacB counter selection marker, an unmarked mini-Tn7 insertion mutant was created. Mini-Tn7 insertion did not affect bacterial virulence on the tested plant. The mini-Tn7 and FLP-FRT systems also work well in Xanthomonas oryzae pv. oryzae. © 2009 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.en_US
dc.rightsMahidol Universityen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleMini-Tn7 vectors as genetic tools for gene cloning at a single copy number in an industrially important and phytopathogenic bacteria, Xanthomonas spp.: Research letteren_US
Appears in Collections:Scopus 2006-2010

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