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dc.contributor.authorAjiraporn Kongpolen_US
dc.contributor.authorThunyarat Pongtharangkulen_US
dc.contributor.authorJunichi Katoen_US
dc.contributor.authorKohsuke Hondaen_US
dc.contributor.authorHisao Ohtakeen_US
dc.contributor.authorAlisa S. Vangnaien_US
dc.contributor.otherChulalongkorn Universityen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherHiroshima Universityen_US
dc.contributor.otherOsaka Universityen_US
dc.identifier.citationFEMS Microbiology Letters. Vol.297, No.2 (2009), 225-233en_US
dc.description.abstractBrevibacillus agri strain 13 was isolated and characterized as a Gram-positive organic-solvent-tolerant bacterium able to grow at 45 °C. It can tolerate high concentrations (5% and 20%, v/v) of various organic solvents with a broad range of log Powwhen the organic solvent was provided as a nonaqueous layer. Although it can tolerate a number of aromatic solvents, it cannot utilize them as a sole carbon source. The surface characteristics of cells exposed to organic solvent were investigated using the bacterial adhesion to hydrocarbon test, a contact angle measurement, ζ potential determination, and fluorescence microscopy analysis and compared with that of nonexposed cells. The results showed that although it has a hydrophilic cell surface, it has a unique indigenous cell surface characteristic in which the cells can stabilize solvent-in-water emulsion by adhering to the solvent-water interface of the solvent droplets. The tolerance and predilection of B. agri strain 13 toward organic solvents may suggest its potential application as a whole-cell biocatalyst for the biotransformation process of water-immiscible substrate(s). © 2009 Federation of European Microbiological Societies.en_US
dc.rightsMahidol Universityen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleCharacterization of an organic-solvent-tolerant brevibacillus agri strain 13 able to stabilize solvent/water emulsionen_US
Appears in Collections:Scopus 2006-2010

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