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Title: Microarray identification of Clostridium difficile core components and divergent regions associated with host origin
Authors: Tavan Janvilisri
Joy Scaria
Angela D. Thompson
Ainsley Nicholson
Brandi M. Limbago
Luis G. Arroyo
J. Glenn Songer
Yrjö T. Gröhn
Yung Fu Chang
Cornell University
National Center for Emerging and Zoonotic Infectious Diseases
Ontario Veterinary College
University of Arizona
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology
Issue Date: 1-Jun-2009
Citation: Journal of Bacteriology. Vol.191, No.12 (2009), 3881-3891
Abstract: Clostridium difficile is a gram-positive, spore-forming enteric anaerobe which can infect humans and a wide variety of animal species. Recently, the incidence and severity of human C. difficile infection has markedly increased. In this study, we evaluated the genomic content of 73 C. difficile strains isolated from humans, horses, cattle, and pigs by comparative genomic hybridization with microarrays containing coding sequences from C. difficile strains 630 and QCD-32g58. The sequenced genome of C. difficile strain 630 was used as a reference to define a candidate core genome of C. difficile and to explore correlations between host origins and genetic diversity. Approximately 16% of the genes in strain 630 were highly conserved among all strains, representing the core complement of functional genes defining C. difficile. Absent or divergent genes in the tested strains were distributed across the entire C. difficile 630 genome and across all the predicted functional categories. Interestingly, certain genes were conserved among strains from a specific host species, but divergent in isolates with other host origins. This information provides insight into the genomic changes which might contribute to host adaptation. Due to a high degree of divergence among C. difficile strains, a core gene list from this study offers the first step toward the construction of diagnostic arrays for C. difficile. Copyright © 2009, American Society for Microbiology. All Rights Reserved.
ISSN: 00219193
Appears in Collections:Scopus 2006-2010

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