Simple jQuery Dropdowns
Please use this identifier to cite or link to this item:
Title: Molecular characterization and analysis of a truncated serotonin receptor gene expressed in neural and reproductive tissues of abalone
Authors: Sasiporn Panasophonkul
Somjai Apisawetakan
Scott F. Cummins
Patrick S. York
Bernard M. Degnan
Peter J. Hanna
Porncharn Saitongdee
Prasert Sobhon
Prapee Sretarugsa
Mahidol University
Deakin University
University of Queensland
Keywords: Biochemistry, Genetics and Molecular Biology;Health Professions;Medicine
Issue Date: 1-May-2009
Citation: Histochemistry and Cell Biology. Vol.131, No.5 (2009), 629-642
Abstract: In molluscs, the neurotransmitter serotonin (5-HT) has been linked to a variety of biological roles including gamete maturation and spawning. The possible involvement of 5-HT in abalone gamete release was demonstrated by a dose-dependent increase in Haliotis rubra gonad contractile bioactivity following 5-HT stimulation. Physiological functions associated with 5-HT, are mediated through binding to 5-HT receptors. A cDNA encoding a putative 5-HT receptor consisting of 359 amino acids was isolated from the tropical abalone H. asinina, termed 5-HT1 ha. The 5-HT1 hashares G-protein-coupled receptor motifs with metazoan 5-HT receptors, including predicted transmembrane domains, active sites for protein kinase action, and N-linked glycosylation sites. However, the third intracellular loop of 5-HT1 hais relatively short, and only six transmembrane domains are predicted, implying a truncated receptor. Phylogenetic analysis with known 5-HT receptor genes suggests that 5-HT1 habelongs to the type 1 5-HT receptor family. Expression analysis by RT-PCR showed that 5-HT1 hamRNA was present in all tissues examined, including the neural ganglia and gonad tissues. Immunocytochemistry revealed the presence of 5-HT1 haspecifically within the soma of neuronal cells located in the outer cortex of both cerebral and pleuropedal ganglia. In ovarian and testicular tissues, 5-HT1 haimmunoreactivity was observed in epithelial cells of the outer capsule and connective tissue of the trabeculae to which the gamete follicles adhere. Whether this receptor transcript is translated to a functional protein needs to be verified, but if so, it could play a role in reproduction. © 2009 Springer-Verlag.
ISSN: 09486143
Appears in Collections:Scopus 2006-2010

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.