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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/27258
Title: Characterization of a regulatory gene, aveR, for the biosynthesis of avermectin in Streptomyces avermitilis
Authors: Shigeru Kitani
Haruo Ikeda
Takako Sakamoto
Satoru Noguchi
Takuya Nihira
Osaka University
Kitasato University
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology
Issue Date: 1-Apr-2009
Citation: Applied Microbiology and Biotechnology. Vol.82, No.6 (2009), 1089-1096
Abstract: Avermectin is an important macrocyclic polyketide produced by Streptomyces avermitilis and widely used as an anthelmintic agent in the medical, veterinary, and agricultural fields. The avermectin biosynthetic gene cluster contains aveR, which belongs to the LAL-family of regulatory genes. In this study, aveR was inactivated by gene replacement in the chromosome of S. avermitilis, resulting in the complete loss of avermectin production. The aveR mutant was unable to convert an avermectin intermediate to any avermectin derivatives, and complementation by intact aveR and its proper upstream region restored avermectin production in the mutant, suggesting that AveR is a positive regulator controlling the expression of both polyketide biosynthetic genes and postpolyketide modification genes in avermectin biosynthesis. Despite the general concept that an increased amount of a positive pathway-specific regulator leads to higher production, a higher amount of aveR resulted in complete loss of avermectin, indicating that there is a maximum threshold concentration of aveR for the production of avermectin. © 2009 Springer-Verlag.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=63949083799&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/27258
ISSN: 01757598
Appears in Collections:Scopus 2006-2010

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