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Title: Human monoclonal ScFv neutralize lethal Thai cobra, Naja kaouthia, neurotoxin
Authors: Kasem Kulkeaw
Yuwaporn Sakolvaree
Potjanee Srimanote
Pongsri Tongtawe
Santi Maneewatch
Nitat Sookrung
Anchalee Tungtrongchitr
Pramuan Tapchaisri
Hisao Kurazono
Wanpen Chaicumpa
Thammasat University
Mahidol University
Obihiro University of Agriculture and Veterinary Medicine
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 6-Mar-2009
Citation: Journal of Proteomics. Vol.72, No.2 (2009), 270-282
Abstract: Animal derived anti-Naja. kaouthia (Thai cobra) venom is used for specific treatment of the snake bitten victims. Many recipients develop allergic reaction or anti-isotype response which causes serum sickness. A better therapeutic antibody is needed. In this study, long α-neurotoxin was purified from the N. kaouthia holovenom and verified by 2D-LC/MS-MS. The toxin was used as antigen in a phage bio-panning to select phage clones displaying human single chain variable antibody fragments (HuScFv) from a phage display antibody library constructed from immunoglobulin genes of non-immunized Thai blood donors. HuScFv that specifically bound to the neurotoxin were produced from huscfv-phagemid transformed E. coli clones and affinity purified. The HuScFv could neutralize toxicity of the N. kaouthia neurotoxin and rescued the envenomized mice from the neurotoxin mediated lethality. Peptide mimotope of the neutralizing HuScFv matched with an amino acid sequence (epitope) located in the loop-3 of the N. kaouthia long α-neurotoxin which functions in acetylcholine receptor binding. The mimotope is also similar to peptide sequences found on other snake venom neurotoxins implying a possibility of the HuScFv to exert pan-neutralizing activity against multiple snake neurotoxins. © 2009 Elsevier B.V. All rights reserved.
ISSN: 18743919
Appears in Collections:Scopus 2006-2010

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