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Title: A novel method and simple apparatus for the detection of thermophilic Campylobacter spp. in chicken meat products
Authors: Sueptrakool Wisessombat
Kanokwan Kittiniyom
Potjanee Srimanote
Wijit Wonglumsom
Supayang P. Voravuthikunchai
Prince of Songkla University
Mahidol University
Thammasat University
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology;Medicine
Issue Date: 1-Feb-2009
Citation: Journal of Microbiological Methods. Vol.76, No.2 (2009), 169-173
Abstract: Conventional procedures for isolation of thermophilic Campylobacter spp. from chicken are complex, labor intensive, and time-consuming. The objective of this study was to create a novel Campylobacter culturing apparatus. A main concept of the device was based on the ability of Campylobacter to pass through a 0.45 μm pore size filter in viscous media. Preliminary study demonstrated that only viable Campylobacter moved through the membrane filter and could multiply in the enrichment culture. C. jejuni, C. coli, C. lari, and C. upsaliensis in the chicken samples were detected at cell concentrations as low as 10 cfu/g, after 24 h incubation at 42 °C. In total, 84 retail chicken samples were comparatively studied using both conventional method and apparatus. Sixteen samples (19.05%) were positive by the apparatus method; 14 (16.66%) of these positive samples contained C. coli and 2 (2.38%) contained C. jejuni. With the conventional method, 7 (8.33%) samples were positive 7 (8.33%) with C. coli. In conclusion, the apparatus detected more positive samples than did the conventional culture method. © 2008 Elsevier B.V. All rights reserved.
ISSN: 01677012
Appears in Collections:Scopus 2006-2010

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