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Title: Preparation and agglutination of immuno-nanolatex for malaria diagnosis
Authors: Duangporn Polpanich
Pramuan Tangboriboonrat
Abdelhamid Elaissari
Mahidol University
Thailand National Science and Technology Development Agency
Universite de Lyon
Laboratoire d'Automatique et de Génie des Procédés
Keywords: Chemical Engineering;Engineering;Materials Science;Medicine;Pharmacology, Toxicology and Pharmaceutics
Issue Date: 1-Oct-2009
Citation: Journal of Biomedical Nanotechnology. Vol.5, No.5 (2009), 486-492
Abstract: This is the first systematic study on the adsorption of malaria antigen onto latex particles to elaborate immuno-latex for diagnostic purpose. Immuno-latex was prepared from adsorption of malaria antigen onto polystyrene (PS) or poly(styrene-co-acrylic acid) (St/AA) latex particles and was used for detection of antibody to P. Falciparum based on agglutination test. Adsorption behavior of the malaria antigen onto polymer particles as a function of incubation time, antigen concentration, pH and concentration of the medium was studied. The prepared immuno-latexes were characterized in terms of immuno-agglutination and specific bridging using patient's plasma with P. Falciparum infection. This evaluation was performed using photon correlation spectroscopy. Copyright © 2009 American Scientific Publishers All rights reserved.
ISSN: 15507041
Appears in Collections:Scopus 2006-2010

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