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Title: Mechanisms of the action of povidone-iodine against human and avian influenza A viruses: Its effects on hemagglutination and sialidase activities
Authors: Nongluk Sriwilaijaroen
Prapon Wilairat
Hiroaki Hiramatsu
Tadanobu Takahashi
Takashi Suzuki
Morihiro Ito
Yasuhiko Ito
Masato Tashiro
Yasuo Suzuki
Faculty of Medicine, Thammasat University
Chubu University
Mahidol University
University of Shizuoka
National Institute of Infectious Diseases
Keywords: Immunology and Microbiology;Medicine
Issue Date: 9-Nov-2009
Citation: Virology Journal. Vol.6, (2009)
Abstract: Background: Influenza virus infection causes significant morbidity and mortality and has marked social and economic impacts throughout the world. The influenza surface glycoproteins, hemagglutinin (HA) and neuraminidase (NA), act cooperatively to support efficient influenza A virus replication and provide the most important targets for anti-influenza chemotherapy. In this study, povidone-iodine (PVP-I), which has a broad-spectrum microbicidal property, was examined for its inhibitory effects against influenza virus infection in MDCK cells and the mechanisms of PVP-I action on HA and NA were revealed. Results: Results obtained using a novel fluorescence- and chromogenic-based plaque inhibition assay showed that 1.56 mg/ml PVP-I inhibited infections in MDCK cells of human (8 strains) and avian (5 strains) influenza A viruses, including H1N1, H3N2, H5N3 and H9N2, from 23.0-97.5%. A sialidase inhibition assay revealed that PVP-I inhibited N1, N2 and N3 neuraminidases with IC50values of 9.5-212.1 g/ml by a mixed-type inhibition mechanism. Receptor binding inhibition and hemagglutinin inhibition assays indicated that PVP-I affected viral hemagglutinin rather than host-specific sialic acid receptors. Conclusion: Mechanisms of reduction of viral growth in MDCK cells by PVP-I involve blockade of viral attachment to cellular receptors and inhibition of viral release and spread from infected cells. Therefore, PVP-I is useful to prevent infection and limit spread of human and avian influenza viruses. © 2009 Sriwilaijaroen et al; licensee BioMed Central Ltd.
ISSN: 1743422X
Appears in Collections:Scopus 2006-2010

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