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Title: Rapid molecular detection of Opisthorchis viverrini in human fecal samples by real-time polymerase chain reaction
Authors: Pewpan M. Intapan
Tongjit Thanchomnang
Viraphong Lulitanond
Phunthira Pongsaskulchoti
Wanchai Maleewong
Khon Kaen University
Mahidol University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Nov-2009
Citation: American Journal of Tropical Medicine and Hygiene. Vol.81, No.5 (2009), 917-920
Abstract: Real-time fluorescence resonance energy transfer (FRET) polymerase chain reaction (PCR) supplemented with melting curve analysis is a highly sensitive and fast method offering a high throughput. We report the development of a real-time FRET PCR for molecular detection of Opisthorchis viverrini in human fecal samples. The diagnostic sensitivity, specificity, accuracy, and positive and negative predictive values of this method were 97.5%, 100%, 98.9%, 100%, and 98.2%, respectively. The sensitivity was not significantly different from that of the quantified formalin-ethyl acetate concentration technique, the gold standard (P > 0.05). The procedure has potential for diagnosis of human opisthorchiasis in disease-endemic areas, for large epidemiologic investigations involving at risk populations, and monitoring eradication programs of the liver fluke, which causes hepatobiliary diseases and induces cholangiocarcinoma. Copyright © 2009 by The American Society of Tropical Medicine and Hygiene.
ISSN: 00029637
Appears in Collections:Scopus 2006-2010

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