Simple jQuery Dropdowns
Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/27697
Title: A role for autophagolysosomes in dengue virus 3 production in HepG2 cells
Authors: Atefeh Khakpoor
Mingkwan Panyasrivanit
Nitwara Wikan
Duncan R. Smith
Mahidol University
Keywords: Immunology and Microbiology
Issue Date: 29-Jun-2009
Citation: Journal of General Virology. Vol.90, No.5 (2009), 1093-1103
Abstract: We have recently proposed that amphisomes act as a site for translation and replication of dengue virus (DENV)-2 and that DENV-2 entry and replication are linked through an ongoing association with membranes of an endosomal-autophagosomal lineage. In this report, we present the results of an investigation into the interaction between DENV-3 and the autophagy machinery. Critically, treatment with the lysosomal fusion inhibitor L-asparagine differentiated the interaction of DENV-3 from that of DENV-2. Inhibition of fusion of autophagosomes and amphisomes with lysosomes resulted in decreased DENV-3 production, implying a role for the autophagolysosome in the DENV-3 life cycle. Evidence based upon the co-localization of LC3 and cathepsin D with double stranded RNA and NS1 protein, as assessed by confocal microscopy, support a model in which DENV-3 interacts with both amphisomes and autophagolysosomes. These results demonstrate that the interactions between DENV and the host cell autophagy machinery are complex and may be determined in part by virus-encoded factors. © 2009 SGM.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=67449086380&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/27697
ISSN: 14652099
00221317
Appears in Collections:Scopus 2006-2010

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.