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dc.contributor.authorAtefeh Khakpooren_US
dc.contributor.authorMingkwan Panyasrivaniten_US
dc.contributor.authorNitwara Wikanen_US
dc.contributor.authorDuncan R. Smithen_US
dc.contributor.otherMahidol Universityen_US
dc.identifier.citationJournal of General Virology. Vol.90, No.5 (2009), 1093-1103en_US
dc.description.abstractWe have recently proposed that amphisomes act as a site for translation and replication of dengue virus (DENV)-2 and that DENV-2 entry and replication are linked through an ongoing association with membranes of an endosomal-autophagosomal lineage. In this report, we present the results of an investigation into the interaction between DENV-3 and the autophagy machinery. Critically, treatment with the lysosomal fusion inhibitor L-asparagine differentiated the interaction of DENV-3 from that of DENV-2. Inhibition of fusion of autophagosomes and amphisomes with lysosomes resulted in decreased DENV-3 production, implying a role for the autophagolysosome in the DENV-3 life cycle. Evidence based upon the co-localization of LC3 and cathepsin D with double stranded RNA and NS1 protein, as assessed by confocal microscopy, support a model in which DENV-3 interacts with both amphisomes and autophagolysosomes. These results demonstrate that the interactions between DENV and the host cell autophagy machinery are complex and may be determined in part by virus-encoded factors. © 2009 SGM.en_US
dc.rightsMahidol Universityen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleA role for autophagolysosomes in dengue virus 3 production in HepG2 cellsen_US
Appears in Collections:Scopus 2006-2010

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