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Title: Co-localization of constituents of the dengue virus translation and replication machinery with amphisomes
Authors: Mingkwan Panyasrivanit
Atefeh Khakpoor
Nitwara Wikan
Duncan R. Smith
Mahidol University
Keywords: Immunology and Microbiology
Issue Date: 27-Apr-2009
Citation: Journal of General Virology. Vol.90, No.2 (2009), 448-456
Abstract: Infections with dengue virus (DENV) are a significant public health concern in tropical and subtropical regions. However, little detail is known about how DENV interacts with the host-cell machinery to facilitate its translation and replication. In DENV-infected HepG2 cells, an increase in the level of LC3-II (microtubule-associated protein 1 light chain 3 form II), the autophagosomal membrane-bound form of LC3, was observed, and LC3 was found to co-localize with dsRNA and DENV NS1 protein, as well as ribosomal protein L28, indicating the presence of at least some of the DENV translation/replication machinery on autophagic vacuoles. Inhibition of fusion of autophagic vacuoles with lysosomes resulted in an increase in both intracellular and extracellular virus, and co-localization observed between mannose-6-phosphate receptor (MPR) and dsRNA and between MPR and LC3 identified the autophagic vacuoles as amphisomes. Amphisomes are formed as a result of fusion between endosomal and autophagic vacuoles, and as such provide a direct link between virus entry and subsequent replication and translation. © 2009 SGM.
ISSN: 14652099
Appears in Collections:Scopus 2006-2010

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