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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/27743
Title: Discrimination of O. viverrini, C. sinensis, H. pumilio and H. taichui using nuclear DNA-based PCR targeting ribosomal DNA ITS regions
Authors: Megumi Sato
Urusa Thaenkham
Paron Dekumyoy
Jitra Waikagul
Mahidol University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Jan-2009
Citation: Acta Tropica. Vol.109, No.1 (2009), 81-83
Abstract: Small liver and minute intestinal flukes are highly prevalent in Southeast Asia, and in mixed infections, their eggs are difficult to differentiate morphologically in fecal samples. PCR assays targeting the ITS regions in ribosomal DNA were designed to identify and differentiate species. The PCR amplicons of Opisthorchis viverrini, Clonorchis sinensis, Haplorchis pumilio, and Haplorchis taichui were 800, 820, 1250, and 930 bp for the ITS1 region, and 380, 390, 380, and 530 bp for ITS2, respectively. The ITS1-region amplicon sizes successfully differentiated 4 species, while only H. taichui were significantly different from the other 3 species in the ITS2 region. PCR assays were employed for preliminary analysis using fecal samples diagnosed as having "small trematode eggs" by modified thick smear, showing 76.2% sensitivity for ITS1 and 95.2% for ITS2. © 2008 Elsevier B.V. All rights reserved.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=57049083447&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/27743
ISSN: 0001706X
Appears in Collections:Scopus 2006-2010

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