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Title: Effects of DHA-enriched hen egg yolk and L-cysteine supplementation on quality of cryopreserved boar semen
Authors: Panida Chanapiwat
Kampon Kaeoket
Padet Tummaruk
Chulalongkorn University
Mahidol University
Keywords: Medicine
Issue Date: 21-Sep-2009
Citation: Asian Journal of Andrology. Vol.11, No.5 (2009), 600-608
Abstract: The objective of the present study was to determine the effects of docosahexaenoic acid (DHA)-enriched hen egg yolks and L-cysteine supplementation on the qualities of the cryopreserved boar semen. A total of 15 ejaculates from 5 Pietrain boars were divided into 4 groups according to the compositions of the freezing extenders used, that is, normal hen egg yolk (group I), DHA-enriched hen egg yolk (group II), normal hen egg yolk with 5 mmol L-1 of cysteine supplementation (group III) and DHA-enriched hen egg yolk with 5 mmol L-1 of cysteine supplementation (group IV). The semen was cryopreserved using controlled rate freezer and was thawed at 50°C for 12 s. Progressive motility, sperm viability, acrosome integrity and functional integrity of sperm plasma membrane of the post-thawed semen were evaluated. The supplementation of L-cysteine in the freezing extender alone (group III) improved progressive motility (P < 0.05), and the supplementation of L-cysteine in combination with DHA-enriched hen egg yolk (group IV) improved both progressive motility (P < 0.05) and acrosome integrity (P < 0.01). The use of DHA-enriched hen egg yolk alone (group II) did not enhance any of the post-thawed semen qualities (P > 0.05). In conclusion, the supplementation of antioxidant L-cysteine alone or in combination with DHA-enriched hen egg yolk significantly improved the post-thawed semen qualities, especially progressive motility and acrosome integrity.
ISSN: 17457262
Appears in Collections:Scopus 2006-2010

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