Simple jQuery Dropdowns
Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorVu Ty Hangen_US
dc.contributor.authorNguyen Minh Nguyeten_US
dc.contributor.authorDinh The Trungen_US
dc.contributor.authorVianney Tricouen_US
dc.contributor.authorSutee Yoksanen_US
dc.contributor.authorNguyen Minh Dungen_US
dc.contributor.authorTran Van Ngocen_US
dc.contributor.authorTran Tinh Hienen_US
dc.contributor.authorJeremy Farraren_US
dc.contributor.authorBridget Willsen_US
dc.contributor.authorCameron P. Simmonsen_US
dc.contributor.otherThe Institute of Science and Technology for Research and Development, Mahidol Universityen_US
dc.contributor.otherUniversity of Oxforden_US
dc.identifier.citationPLoS Neglected Tropical Diseases. Vol.3, No.1 (2009)en_US
dc.description.abstractBackground: Dengue is a public health problem in many countries. Rapid diagnosis of dengue can assist patient triage and management. Detection of the dengue viral protein, NS1, represents a new approach to dengue diagnosis. Methodology/Principal Findings: The sensitivity and specificity of the Platelia NS1 ELISA assay and an NS1 lateral flow rapid test (LFRT) were compared against a gold standard reference diagnostic algorithm in 138 Vietnamese children and adults. Overall, the Platelia NS1 ELISA was modestly more sensitive (82%) than the NS1 LFRT (72%) in confirmed dengue cases. Both ELISA and LFRT assays were more sensitive for primary than secondary dengue, and for specimens collected within 3 days of illness onset relative to later time points. The presence of measurable DENV-reactive IgG and to a lesser extent IgM in the test sample was associated with a significantly lower rate of NS1 detection in both assays. NS1 positivity was associated with the underlying viraemia, as NS1-positive samples had a significantly higher viraemia than NS1-negative samples matched for duration of illness. The Platelia and NS1 LFRT were 100% specific, being negative in all febrile patients without evidence of recent dengue, as well as in patients with enteric fever, malaria, Japanese encephalitis and leptospirosis. Conclusions/Significance: Collectively, these data suggest NS1 assays deserve inclusion in the diagnostic evaluation of dengue patients, but with due consideration for the limitations in patients who present late in their illness or have a concomitant humoral immune response. © 2009 Ty Hang et al.en_US
dc.rightsMahidol Universityen_US
dc.titleDiagnostic accuracy of NS1 ELISA and lateral flow rapid tests for dengue sensitivity, specificity and relationship to viraemia and antibody responsesen_US
Appears in Collections:Scopus 2006-2010

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.