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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/28178
Title: Expression and purification of 30 kilodalton protein antigen of ARA <sup>-</sup>Burkholderia pseudomallei
Authors: Utane Rungpanich
Supinya Pongsunk
Takayuki Ezaki
Pattama Ekpo
Mahidol University
Srinakharinwirot University
Gifu University
Keywords: Medicine
Issue Date: 1-Mar-2009
Citation: Southeast Asian Journal of Tropical Medicine and Public Health. Vol.40, No.2 (2009), 295-301
Abstract: The 30 kDa protein of B. pseudomallei is found in virulent Ara- but not avirulent Ara+ strain. The gene was cloned in Escherichia coli JM105 employing pInIII-C2 vector. The open reading frame was 870 nucleotides with a guanine plus cytosine content of 69.9%. Arginine was the most abundant amino acid in the protein, having a PI of 12.65. Nucleotide sequence of the gene was 96% identical to B. pseudomallei 1710b chromosome II sequence CP000125.1, encoding an oxidoreductase of the short chain dehydrogenase/ reductase family. The 30 kDa antigen was expressed as a maltose-fusion protein with a yield of 5.25 mg/l of bacterial culture.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=63149121299&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/28178
ISSN: 01251562
Appears in Collections:Scopus 2006-2010

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