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Title: Cloning, characterization, and expression of Vitelline Protein Bi and its encoding gene in the Liver Fluke, Fasciola gigantica
Authors: Kritsana Homwutthiwong
Ardool Meepool
Rudi Grams
Chaitip Wanichanon
Vithoon Viyanant
Prasert Sobhon
Mahidol University
Burapha University
Thammasat University
Keywords: Medicine
Issue Date: 1-Mar-2009
Citation: Southeast Asian Journal of Tropical Medicine and Public Health. Vol.40, No.2 (2009), 199-210
Abstract: A cDNA containing a 813 bp open reading frame encoding vitelline protein BI (FgVPBI) of Fasciola gigantica was cloned. FgVPBI has 96% sequence identity with VPBI of Fasciola hepatica and 84% identity with VPBIIF. hepatica. It is far less similar to eggshell precursor proteins of other trematode species, for example, 29% identity with C. sinensis. Northern blot hybridization of total RNA from adult parasites demonstrated a FgVPBI transcript with a size of 1,000 nucleotides. FgVPBI mRNA is localized in the vitelline cells in both vitelline glands and intrauterine eggs. Recombinant FgVPBI was expressed as a 31.5 kDa protein in Escherichia coli and used for production of a polyclonal antiserum in rabbits. The FgVPBI antiserum detected immunoblotted rFgVPBI and native eggshell precursor protein at molecular weights of 31.5 kDa and 31 kDa, respectively. Immunolocalization showed strong staining in the cytoplasm of vitelline cells, in eggshell globules and the shells of eggs.
ISSN: 01251562
Appears in Collections:Scopus 2006-2010

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