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dc.contributor.authorChawalit Ngernsombaten_US
dc.contributor.authorSuthasinee Sreesaien_US
dc.contributor.authorPhurt Harnvoravongchaien_US
dc.contributor.authorSurang Chankhamhaengdechaen_US
dc.contributor.authorTavan Janvilisrien_US
dc.contributor.otherMahidol University. Faculty of Science. Department of Biochemistryen_US
dc.contributor.otherMahidol University. Faculty of Science. Department of Biologyen_US
dc.date.accessioned2017-10-02T11:37:11Z-
dc.date.available2017-10-02T11:37:11Z-
dc.date.created2017-10-02-
dc.date.issued2017-
dc.identifier.citationScientific Reports. Vol.7, No.1 (2017), 9982en_US
dc.identifier.urihttp://repository.li.mahidol.ac.th/dspace/handle/123456789/2835-
dc.description.abstractClostridium difficile is a major cause of antibiotic-associated diarrhea and the treatment thereof becomes more difficult owing to a rise of multidrug resistant strains. ATP-binding cassette (ABC) transporters are known to play a crucial role in the resistance to multiple antibiotics. In this study, the potential contribution of an ABC transporter in C. difficile multidrug resistance was investigated. The expression level of the cd2068 gene in C. difficile encoding an ABC transporter was up-regulated following the exposure to certain antibiotics compared to the control cells. Heterologous expression of CD2068 in Escherichia coli revealed that it mediated the efflux of fluorescent substrates and conferred resistance to multiple drugs. The CD2068-associated ATPase activity in membrane vesicles was also stimulated by various antibiotics. Furthermore, the insertional inactivation of the cd2068 gene in C. difficile led to a significant increase in susceptibility to antibiotics, which could be genetically complemented, supporting that CD2068 was directly associated to the drug resistance. These results demonstrate the potential role for the ABC transporter CD2068 in the resistance mechanism against multiple drugs in C. difficile.en_US
dc.language.isoenen_US
dc.rightsMahidol Universityen_US
dc.subjectClostridium difficileen_US
dc.subjectCD2068en_US
dc.subjectmultiple drugsen_US
dc.subjectantibioticsen_US
dc.subjectmultiple antibioticsen_US
dc.subjectOpen Access articleen_US
dc.titleCD2068 potentially mediates multidrug efflux in Clostridium difficileen_US
dc.typeResearch Articleen_US
dc.rights.holderScopusen_US
dc.identifier.doi10.1038/s41598-017-10155-x-
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