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dc.contributor.authorPhilip L. Felgneren_US
dc.contributor.authorMatthew A. Kayalaen_US
dc.contributor.authorAdam Vigilen_US
dc.contributor.authorChad Burken_US
dc.contributor.authorRie Nakajima-Sasakien_US
dc.contributor.authorJozelyn Pabloen_US
dc.contributor.authorDouglas M. Molinaen_US
dc.contributor.authorSiddiqua Hirsten_US
dc.contributor.authorJanet S.W. Chewen_US
dc.contributor.authorDongling Wangen_US
dc.contributor.authorGladys Tanen_US
dc.contributor.authorMelanie Duffielden_US
dc.contributor.authorRon Yangen_US
dc.contributor.authorJulien Neelen_US
dc.contributor.authorNarisara Chantratitaen_US
dc.contributor.authorGreg Bancroften_US
dc.contributor.authorGanjana Lertmemongkolchaien_US
dc.contributor.authorD. Huw Daviesen_US
dc.contributor.authorPierre Baldien_US
dc.contributor.authorSharon Peacocken_US
dc.contributor.authorRichard W. Titballen_US
dc.contributor.otherUniversity of California, Irvineen_US
dc.contributor.otherAntigen Discovery Inc.en_US
dc.contributor.otherDSO National Laboratoriesen_US
dc.contributor.otherDefence Science and Technology Laboratoryen_US
dc.contributor.otherUniversity of Exeteren_US
dc.contributor.otherLondon School of Hygiene & Tropical Medicineen_US
dc.contributor.otherKhon Kaen Universityen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherNuffield Department of Clinical Medicineen_US
dc.identifier.citationProceedings of the National Academy of Sciences of the United States of America. Vol.106, No.32 (2009), 13499-13504en_US
dc.description.abstractUnderstanding the way in which the immune system responds to infection is central to the development of vaccines and many diagnostics. To provide insight into this area, we fabricated a protein microarray containing 1,205 Burkholderia pseudomallei proteins, probed it with 88 melioidosis patient sera, and identified 170 reactive antigens. This subset of antigens was printed on a smaller array and probed with a collection of 747 individual sera derived from 10 patient groups including melioidosis patients from Northeast Thailand and Singapore, patients with different infections, healthy individuals from the USA, and from endemic and nonendemic regions of Thailand. We identified 49 antigens that are significantly more reactive in melioidosis patients than healthy people and patients with other types of bacterial infections. We also identified 59 cross-reactive antigens that are equally reactive among all groups, including healthy controls from the USA. Using these results we were able to devise a test that can classify melioidosis positive and negative individuals with sensitivity and specificity of 95% and 83%, respectively, a significant improvement over currently available diagnostic assays. Half of the reactive antigens contained a predicted signal peptide sequence and were classified as outer membrane, surface structures or secreted molecules, and an additional 20% were associated with pathogenicity, adaptation or chaperones. These results show that microarrays allow a more comprehensive analysis of the immune response on an antigen-specific, patient-specific, and population-specific basis, can identify serodiagnostic antigens, and contribute to a more detailed understanding of immunogenicity to this pathogen.en_US
dc.rightsMahidol Universityen_US
dc.titleA Burkholderia pseudomallei protein microarray reveals serodiagnostic and cross-reactive antigensen_US
Appears in Collections:Scopus 2006-2010

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