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Title: Analysis of ultra low genome conservation in Clostridium difficile
Authors: Joy Scaria
Lalit Ponnala
Tavan Janvilisri
Weiwei Yan
Lukas A. Mueller
Yung Fu Chang
Cornell University
Mahidol University
Boyce Thompson Institute for Plant Research
Keywords: Agricultural and Biological Sciences;Biochemistry, Genetics and Molecular Biology
Issue Date: 20-Dec-2010
Citation: PLoS ONE. Vol.5, No.12 (2010)
Abstract: Microarray-based comparative genome hybridisations (CGH) and genome sequencing of Clostridium difficile isolates have shown that the genomes of this species are highly variable. To further characterize their genome variation, we employed integration of data from CGH, genome sequencing and putative cellular pathways. Transcontinental strain comparison using CGH data confirmed the emergence of a human-specific hypervirulent cluster. However, there was no correlation between total toxin production and hypervirulent phenotype, indicating the possibility of involvement of additional factors towards hypervirulence. Calculation of C. difficile core and pan genome size using CGH and sequence data estimated that the core genome is composed of 947 to 1,033 genes and a pan genome comprised of 9,640 genes. The reconstruction, annotation and analysis of cellular pathways revealed highly conserved pathways despite large genome variation. However, few pathways such as tetrahydrofolate biosynthesis were found to be variable and could be contributing to adaptation towards virulence such as antibiotic resistance. © 2010 Scaria et al.
ISSN: 19326203
Appears in Collections:Scopus 2006-2010

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