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Title: BurkDiff: A real-time PCR allelic discrimination assay for burkholderia Pseudomallei and B. mallei
Authors: Jolene R. Bowers
David M. Engelthaler
Jennifer L. Ginther
Talima Pearson
Sharon J. Peacock
Apichai Tuanyok
David M. Wagner
Bart J. Currie
Paul S. Keim
Translational Genomics Research Institute
Northern Arizona University
Mahidol University
Menzies School of Health Research
Keywords: Agricultural and Biological Sciences;Biochemistry, Genetics and Molecular Biology;Medicine
Issue Date: 9-Dec-2010
Citation: PLoS ONE. Vol.5, No.11 (2010)
Abstract: A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of B. mallei, and 390 isolates of clinically relevant non-target species. Concordance of results with traditional speciation methods and no cross-reactivity to non-target species show BurkDiff is a robust, highly validated assay for the detection and differentiation of B. pseudomallei and B. mallei. © 2010 Bowers et al.
ISSN: 19326203
Appears in Collections:Scopus 2006-2010

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