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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/28508
Title: Growth and isoflavonoid accumulation of Pueraria candollei var. candollei and P. candollei var. mirifica cell suspension cultures
Authors: Panitch Boonsnongcheep
Sirintra Korsangruang
Noppamas Soonthornchareonnon
Yupyn Chintapakorn
Promchit Saralamp
Sompop Prathanturarug
Mahidol University
Chulalongkorn University
Keywords: Agricultural and Biological Sciences
Issue Date: 1-May-2010
Citation: Plant Cell, Tissue and Organ Culture. Vol.101, No.2 (2010), 119-126
Abstract: We established cell suspension cultures derived from leaf, stem, and root calli of Pueraria candollei var. candollei and P. candollei var. mirifica using liquid Murashige and Skoog (MS) medium supplemented with 0.56 μM 6-benzyladenine (BA) and 4.52 μM 2,4-dichlorophenoxyacetic acid (2,4-D). Growth of the cell suspension cultures progressed to the stationary phase within 15-24 days. Methanolic extracts of cell suspension cultures of both varieties of P. candollei were analyzed using a validated HPLC protocol. All cell lines derived from leaf, stem, and root explants produced four major isoflavonoids: daidzein, daidzin, genistein, and genistin; these isoflavonoids were detected only in the roots of intact plants. Furthermore, the isoflavonoid contents of the cell suspension cultures were higher than those of intact plants. Thus, cell suspension culture of both varieties of P. candollei may be an effective tool for isoflavonoid production. © 2010 Springer Science+Business Media B.V.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77952010989&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/28508
ISSN: 01676857
Appears in Collections:Scopus 2006-2010

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