Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/28539
Title: Expression and characterization of three new glutathione transferases, an epsilon (AcGSTE2-2), Omega (AcGSTO1-1), and Theta (AcGSTT1-1) from anopheles cracens (Diptera: Culicidae), a major thai malaria vector
Authors: Jeerang Wongtrakul
Saengtong Pongjaroenkit
Posri Leelapat
Woottichai Nachaiwieng
La Aied Prapanthadara
Albert J. Ketterman
Chiang Mai University
Maejo University
Mahidol University
Keywords: Agricultural and Biological Sciences;Immunology and Microbiology;Medicine
Issue Date: 1-Feb-2010
Citation: Journal of Medical Entomology. Vol.47, No.2 (2010), 162-171
Abstract: Glutathione transferases (GSTs) (E.C.2.5.1.18) are multifunctional enzymes involved in the detoxification of many exogenous and endogenous compounds. This study aimed to characterize several new GSTs from Anopheles cracens, a major Thai malaria vector formerly known as Anopheles dirus. The three recombinant enzymes obtained were from the epsilon, theta and omega classes. They showed 8093% identity to orthologous An. gambiae GSTs. AcGSTE2-2 possessed peroxidase activity that cannot be detected for the An. gambiae AgGSTE2-2. AcGSTT1-1 had high activity toward several substrates that are specific for mammalian theta class. The AcGSTO1-1 can use 1-chloro-2,4-dinitrobenzene, dichloroacetic acid, and hydroxyethyl disulfide substrates. The enzymes bound but did not metabolize the organophosphate temephos. The epsilon AcGSTE2-2 functioned as a peroxidase and DDT metabolizing enzyme. The theta AcGSTT1-1 functioned not only as peroxidase but also acted as a binding protein for organophosphates. The omega GST had thiol transferase activity suggesting a role in oxidative stress response. © 2010 Entomological Society of America.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77949302356&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/28539
ISSN: 00222585
Appears in Collections:Scopus 2006-2010

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