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|Title:||Structural evidence of glycoprotein assembly in cellular membrane compartments prior to alphavirus budding|
Juan M. Espinoza
Susan W. Barnett
Indresh K. Srivastava
R. Holland Cheng
University of California, Davis
Wadsworth Center for Laboratories and Research
Japan Electron Optics Laboratory System and Technology Co.
Novartis Vaccines and Diagnostics, Inc.
|Keywords:||Agricultural and Biological Sciences;Immunology and Microbiology|
|Citation:||Journal of Virology. Vol.84, No.21 (2010), 11145-11151|
|Abstract:||Membrane glycoproteins of alphavirus play a critical role in the assembly and budding of progeny virions. However, knowledge regarding transport of viral glycoproteins to the plasma membrane is obscure. In this study, we investigated the role of cytopathic vacuole type II (CPV-II) through in situ electron tomography of alphavirus-infected cells. The results revealed that CPV-II contains viral glycoproteins arranged in helical tubular arrays resembling the basic organization of glycoprotein trimers on the envelope of the mature virions. The location of CPV-II adjacent to the site of viral budding suggests a model for the transport of structural components to the site of budding. Thus, the structural characteristics of CPV-II can be used in evaluating the design of a packaging cell line for replicon production. Copyright © 2010, American Society for Microbiology. All Rights Reserved.|
|Appears in Collections:||Scopus 2006-2010|
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