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Title: Engineering of a novel chimera of superoxide dismutase and Vitreoscilla Hemoglobin for rapid detoxification of reactive oxygen species
Authors: Chartchalerm Isarankura-Na-Ayudhya
Sakda Yainoy
Tanawut Tantimongcolwat
Leif Bülow
Virapong Prachayasittikul
Mahidol University
Lunds Universitet
Keywords: Biochemistry, Genetics and Molecular Biology;Chemical Engineering;Immunology and Microbiology
Issue Date: 1-Dec-2010
Citation: Journal of Bioscience and Bioengineering. Vol.110, No.6 (2010), 633-637
Abstract: The genes encoding human manganese superoxide dismutase (MnSOD) and Vitreoscilla hemoglobin (VHb) were fused in-frame to generate a bifunctional enzyme that possessed MnSOD and peroxidase-like activities. At neutral pH, the coupling of the SOD and peroxidase reactions revealed that the bifunctional enzyme exhibited a 2.5 times shorter transient period and a 1.67 times higher reaction rate at steady-state conditions. Furthermore, the catalytic rate of the bifunctional enzyme was not affected as much by the external H2O2scavenger catalase. This indicates that the bifunctional protein possesses a greater antioxidant capability, which is possibly due to the close proximity between the active site of MnSOD and the heme moiety of VHb. Our findings not only provide insight into the synergistic functions of SOD and peroxidase but also could potentially be used to develop novel therapeutic agents with more efficient O2carrying capability. © 2010 The Society for Biotechnology, Japan.
ISSN: 13891723
Appears in Collections:Scopus 2006-2010

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