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Title: Vanillin enhances TRAIL-induced apoptosis in cancer cells through inhibition of NF-κB activation
Authors: Kriengsak Lirdprapamongkol
Hiroaki Sakurai
Shunsuke Suzuki
Keiichi Koizumi
Orawin Prangsaengtong
Amornrat Viriyaroj
Somsak Ruchirawat
Jisnuson Svasti
Ikuo Saiki
Chulabhorn Research Institute
University of Toyama
Srinakharinwirot University
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Pharmacology, Toxicology and Pharmaceutics
Issue Date: 1-Jul-2010
Citation: In Vivo. Vol.24, No.4 (2010), 501-506
Abstract: Background: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a promising anticancer agent which selectively kills cancer cells with little effect on normal cells. However, TRAIL resistance is widely found in cancer cells. We have previously reported antimetatstatic and antiangiogenic effects of vanillin, a flavoring agent from vanilla. Here we have evaluated the sensitizing effect of vanillin on a TRAIL-resistant human cervical cancer cell line, HeLa. Materials and Methods: Cell viability after treatments was determined by the WST-1 cell counting kit. Apoptosis was demonstrated by detection of caspase-3 activation and cleavage of poly (ADP-ribose) polymerase using immunoblot analysis. Effect of treatments on TRAIL signaling pathway and nuclear factor κB (FN-κB) activation was studied using immunoblot analysis and luciferase reporter assay. Results: Pretreatment of HeLa cells with vanillin enhanced TRAIL-induced cell death through the apoptosis pathway. Vanillin pretreatment inhibited TRAIL-induced phosphorylation of p65 and transcriptional activity of NF-κB. Conclusion: Vanillin sensitizes HeLa cells to TRAIL-induced apoptosis by inhibiting NF-κB activation.
ISSN: 0258851X
Appears in Collections:Scopus 2006-2010

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