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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/28744
Title: Modulation of antioxidant defense by Alpinia galanga and Curcuma aromatica extracts correlates with their inhibition of UVA-induced melanogenesis
Authors: Uraiwan Panich
Kamolratana Kongtaphan
Tassanee Onkoksoong
Kannika Jaemsak
Rattana Phadungrakwittaya
Athiwat Thaworn
Pravit Akarasereenont
Adisak Wongkajornsilp
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Environmental Science;Pharmacology, Toxicology and Pharmaceutics
Issue Date: 1-Apr-2010
Citation: Cell Biology and Toxicology. Vol.26, No.2 (2010), 103-116
Abstract: Ultraviolet A (UVA) irradiation is suggested to contribute to melanogenesis through promoting cellular oxidative stress and impairing antioxidant defenses. An overproduction of melanin can be associated with melanoma skin cancer and hyperpigmentation. Therefore, developing effective antimelanogenic agents is of importance. Alpinia galanga (AG) and Curcuma aromatica (CA) are traditional medicinal plants widely used for skin problems. Hence, this study investigated the antimelanogenic effects of AG and CA extracts (3.8-30 μg/ml) by assessing tyrosinase activity, tyrosinase mRNA levels, and melanin content in human melanoma cells (G361) exposed to UVA. The roles in protecting against melanogenesis were examined by evaluating their inhibitory effects on UVA-induced cellular oxidative stress and modulation of antioxidant defenses including antioxidant enzymes, catalase (CAT) and glutathione peroxidase (GPx), and intracellular glutathione (GSH). In addition, possible active compounds accountable for biological activities of the extracts were identified by thin layer chromatography (TLC)-densitometric analysis. Our study demonstrated that UVA (8 J/cm2) induced both tyrosinase activity and mRNA levels and UVA (16 J/cm2)-mediated melanin production were suppressed by the AG or CA extracts at noncytotoxic concentrations. Both extracts were able to protect against UVA-induced cellular oxidant formation and depletion of CAT and GPx activities and GSH content in a dose-dependent manner. Moreover, TLC-densitometric analysis detected the presence of eugenol and curcuminoids in AG and CA, respectively. This is the first report representing promising findings on AG and CA extract-derived antityrosinase properties correlated with their antioxidant potential. Inhibiting cellular oxidative stress and improving antioxidant defenses might be the mechanisms by which the extracts yield the protective effects on UVA-dependent melanogenesis. © 2009 Springer Science+Business Media B.V.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77952241571&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/28744
ISSN: 07422091
Appears in Collections:Scopus 2006-2010

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