Simple jQuery Dropdowns
Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/28763
Title: A simple, sensitive and green bienzymatic UV-spectrophotometric assay of amoxicillin formulations
Authors: Theerasak Rojanarata
Praneet Opanasopit
Tanasait Ngawhirunpat
Choedchai Saehuan
Suthep Wiyakrutta
Vithaya Meevootisom
Silpakorn University
Naresuan University
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology
Issue Date: 5-Mar-2010
Citation: Enzyme and Microbial Technology. Vol.46, No.3-4 (2010), 292-296
Abstract: A simple, fast, sensitive and inexpensive UV-spectrophotometric method for the determination of amoxicillin in pharmaceutical preparations has been developed based on two enzymatic reactions. In this method, d-4-hydroxyphenylglycine side chain of amoxicillin was selectively cleaved off by penicillin acylase. Subsequently, it was reacted with 2-oxoglutarate, by the catalysis of d-phenylglycine aminotransferase, to yield the product with high UV absorption namely 4-hydroxybenzoylformate. The amount of amoxicillin was then determined as a change in absorbance at 335 nm. In this work, the assay conditions were studied and optimized and the method was validated. The calibration curve presented an excellent linearity with r2of 0.9998 (0-100 μM amoxicillin). Detection and quantitation limits were 0.77 and 2.55 μM, respectively. Good accuracy and precision were obtained when the method was tested with amoxicillin capsules and powder for oral suspension. No interference from common excipients in the formulations or degradation products was observed. Finally, since all procedures were performed without the use of any organic solvents or hazardous chemicals which were detrimental to the environment and had a low consumption of reagents, this proposed assay was an ideal green analytical method suitable for the quality control of amoxicillin in pharmaceuticals. © 2009 Elsevier Inc. All rights reserved.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=74149087628&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/28763
ISSN: 01410229
Appears in Collections:Scopus 2006-2010

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.