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dc.contributor.authorTheerasak Rojanarataen_US
dc.contributor.authorPraneet Opanasopiten_US
dc.contributor.authorTanasait Ngawhirunpaten_US
dc.contributor.authorChoedchai Saehuanen_US
dc.contributor.authorSuthep Wiyakruttaen_US
dc.contributor.authorVithaya Meevootisomen_US
dc.contributor.otherSilpakorn Universityen_US
dc.contributor.otherNaresuan Universityen_US
dc.contributor.otherMahidol Universityen_US
dc.identifier.citationEnzyme and Microbial Technology. Vol.46, No.3-4 (2010), 292-296en_US
dc.description.abstractA simple, fast, sensitive and inexpensive UV-spectrophotometric method for the determination of amoxicillin in pharmaceutical preparations has been developed based on two enzymatic reactions. In this method, d-4-hydroxyphenylglycine side chain of amoxicillin was selectively cleaved off by penicillin acylase. Subsequently, it was reacted with 2-oxoglutarate, by the catalysis of d-phenylglycine aminotransferase, to yield the product with high UV absorption namely 4-hydroxybenzoylformate. The amount of amoxicillin was then determined as a change in absorbance at 335 nm. In this work, the assay conditions were studied and optimized and the method was validated. The calibration curve presented an excellent linearity with r2of 0.9998 (0-100 μM amoxicillin). Detection and quantitation limits were 0.77 and 2.55 μM, respectively. Good accuracy and precision were obtained when the method was tested with amoxicillin capsules and powder for oral suspension. No interference from common excipients in the formulations or degradation products was observed. Finally, since all procedures were performed without the use of any organic solvents or hazardous chemicals which were detrimental to the environment and had a low consumption of reagents, this proposed assay was an ideal green analytical method suitable for the quality control of amoxicillin in pharmaceuticals. © 2009 Elsevier Inc. All rights reserved.en_US
dc.rightsMahidol Universityen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleA simple, sensitive and green bienzymatic UV-spectrophotometric assay of amoxicillin formulationsen_US
Appears in Collections:Scopus 2006-2010

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