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Title: Control of secondary metabolism by farX, which is involved in the γ-butyrolactone biosynthesis of Streptomyces lavendulae FRI-5
Authors: Shigeru Kitani
Masashi Doi
Tomohito Shimizu
Asa Maeda
Takuya Nihira
Osaka University
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology
Issue Date: 1-Mar-2010
Citation: Archives of Microbiology. Vol.192, No.3 (2010), 211-220
Abstract: The γ-butyrolactone signaling system is distributed widely among streptomycetes as an important regulatory mechanism of antibiotic production and/or morphological differentiation. IM-2 [(2R,3R,1′R)-2-(1′- hydroxybutyl)-3- hydroxymethyl-γ-butanolide] is a γ-butyrolactone that switches off the production of D-cycloserine but switches on the production of several nucleoside antibiotics as well as blue pigment in Streptomyces lavendulae FRI-5. farX is a member of the afsA-family genes, which are proposed to encode enzymes involved in γ-butyrolactone biosynthesis. Disruption of farX caused overproduction of D-cycloserine, and abolished production of nucleoside antibiotic and blue pigment with the loss of IM-2 production. The Wnding that all phenotypic changes observed in the farX disruptant were restored by the addition of exogenous IM-2 suggested that FarX plays a biosynthetic role in IM-2 production. Transcriptional comparison between the wild-type strain and the farX disruptant revealed that, in addition to already known genes farR1 and farR2, several other genes (farR4, farD, and farE) are under the transcriptional regulation of IM-2. Furthermore, the fact that farX transcription is under the control of IM-2 suggested that S. lavendulae FRI-5 has a fine-tuning system to control γ-butyrolactone production. © Springer-Verlag 2010.
ISSN: 03028933
Appears in Collections:Scopus 2006-2010

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