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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/28778
Title: Monodon baculovirus (MBV) infects the freshwater prawn Macrobrachium rosenbergii cultivated in Thailand
Authors: Warachin Gangnonngiw
Kesinee Laisutisan
Siriporn Sriurairatana
Saengchan Senapin
Niti Chuchird
Chalor Limsuwan
Parin Chaivisuthangkura
Timothy W. Flegel
Mahidol University
Thailand National Science and Technology Development Agency
Kasetsart University
Srinakharinwirot University
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology;Medicine
Issue Date: 1-Mar-2010
Citation: Virus Research. Vol.148, No.1-2 (2010), 24-30
Abstract: Field specimens of post-larvae of the giant freshwater prawn (Macrobrachium rosenbergii) from Thailand showed hepatopancreatic tubule epithelial cells that contained central, eosinophilic inclusions within enlarged nuclei and marginated chromatin. These inclusions resembled those produced by some baculoviruses prior to formation of occlusion bodies that enclose virions in a polyhedrin protein matrix. By electron microscopy, the intranuclear inclusions contained bacilliform, enveloped virions (approximately 327 ± 29 nm × 87 ± 12 nm) with evenly dense, linear nucleocapsids surrounded by trilaminar envelopes with lateral pockets containing nucleoproteinic filaments. In some cases, these were accompanied by moderately electron dense, spherical particles of approximately 20 nm diameter resembling polyhedrin subunits of occlusion bodies (OB) of a bacilliform virus of the black tiger shrimp Penaeus monodon, previously reported from Thailand and called monodon baculovirus (MBV). It is currently listed by the International Committee on Taxonomy of viruses as Penaeus monodon nucleopolyhedrovirus (PemoNPV). Two polymerase chain reaction (PCR) assays for MBV gave positive results with DNA extracts prepared from M. rosenbergii samples using the hot phenol technique. One of these assays targeted the polyhedrin gene of MBV to which the resulting amplicon showed 100% sequence identity. Presence of the Penaeus monodon virus polyhedrin gene was confirmed by in situ hybridization assays and by positive immunohistochemical reactions in one sample batch. The data revealed that MBV can be found but may rarely produce polyhedrin occlusion bodies in M. rosenbergii. © 2009 Elsevier B.V. All rights reserved.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=75449111107&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/28778
ISSN: 01681702
Appears in Collections:Scopus 2006-2010

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